Abberior Instruments


Molecule off
Molecule on (fluorescent)
Exposure area to the switching beam
Fluorescence area of individual molecule
Localization of individual molecule

The basic principle of PALM and STORM suggests that after all molecules are prepared in the off state, a small subset of individual single molecules are stochastically switched into the on state. Typically, both states are realized via a photoizomerisation between molecular states (e.g. cis/trans isomerization)

After nanoscale precise readout of the localization of each individual molecule they are switched back to the dark state or bleached with the readout light. Sequential readout of stochastically switched molecule subsets results in a high resolution image of the labels.

PALM and STORM implement a stochastic readout of the molecule positions. As such the image is taken iteratively by operlapping all identified positions.

On the implementation side a PALM or STORM microscope is quite simple: a widefield-setup with a single-photon sensitive area detector.

The PALM and STORM method provides a theoretically unlimited localization accuracy of individual dye (clusters). The localization accuracy is depending on the number of photons (m) being emitted by one dye (cluster):

where Δx denotes the localization accuracy of each individual molecule, and λ denotes the excitation wavelength.

To deliver a high-resolution image the PALM and STORM concept must leverage numerical methods to calculate and overlay the identified positions of individual molecules.

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