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PALM vs STORM vs ?
PALM and STORM are often used as synonyms, and in fact they have a lot in common. But there are slight differences that can be important for your application. And then there are other superresolution techniques, too – like STED and MINFLUX.
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Lasers in fluorescence microscopy
Today’s high-end fluorescence microscopy is unthinkable without lasers. Reason enough to take a closer look at these sophisticated light sources.
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FLEXPOSURE adaptive illumination
Every technique that allows to observe cells is more or less invasive and fluorescence microscopy is no exception. Many imaging situations profit from a reduction in light dose as provided by FLEXPOSURE adaptive illumination.
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MATRIX STED – many eyes see more than one
MATRIX STED is the next level of STED microscopy – combining superior resolution with outstanding signal quality and clarity.
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MINFLUX – unrivaled spatio-temporal resolution
MINFLUX reaches unprecedented spatio-temporal resolution in light microscopy and provides 2D and 3D localization precisions in the single-digit nanometer range.
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HaloX labeling protocol
Your HaloTag® fusion proteins within living cells, on cell surfaces and on fixed cells can be reversibly colored with the abberior LIVE HaloX® labels. In a PAINT-like process imaging of the sample in an extended periods of time is easily possible, as bleaching are no longer a decisive factor.
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abberior LIVE HaloX
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Why do superresolution microscopists love alpacas?
It is a very simple yet very important fact: the localization precision of any superresolution microscope can only be as good as the size of the fluorescent staining allows. In other words, when your fluorescent dye is too big or too far away from the protein you want to label, you will never be able to reach a resolution that is higher than this offset. The good news is: there are ways to reduce the offset between target protein and fluorescent label. And one of these are nanobodies.
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Let the cells shine with immunofluorescence labeling
The most versatile and therefore most common strategy to bring the dye to the sample is immunofluorescence. In case you always wanted to know how immunofluorescence works and which properties of antibodies make it so powerful and at the same time define its limits!
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STED and virology
A little insight into the advances in virus research made possible by STED microscopy and a hint to were the journey might go.
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Superresolution for biology: when size, time, and context matter
The spatial resolution achievable with today’s light microscopes has unveiled life at the scale of individual molecules. Size is no longer a barrier to seeing biology at the most fundamental level. But life is not static. It emerges from movement and change. How do superresolution technologies hold up to the challenges of documenting dynamic biological mechanisms?
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abberior STAR nanobody
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abberior special
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abberior LIVE special
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abberior FLUX special
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abberior STAR special
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English version of the Spektrum interview with Nobel Laureate Stefan W. Hell about STED, MINFLUX and PALM/STORM.
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Spektrum interview with Nobel Laureate Stefan W. Hell about STED, MINFLUX and PALM/STORM.
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New bioisosteric sulphur-containing choline kinase inhibitors with a tracked mode of action
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Pillarpage
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Coding variants identified in diabetic patients alter PICK1 BAR domain function in insulin granule biogenesis
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Nonspecific binding of common anti-CFTR antibodies in ciliated cells of human airway epithelium
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Super-resolution microscopy to visualize and quantify protein microstructural organization in food materials and its relation to rheology: Egg white proteins
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New GM1 ganglioside derivatives for selective single and double labelling of the natural glycosphingolipid skeleton
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Anti-Siglec-1 antibodies block Ebola viral uptake and decrease cytoplasmic viral entry
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Dissemination of Mycobacterium tuberculosis is associated to a SIGLEC1 null variant that limits antigen exchange via trafficking extracellular vesicles
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Biocompatible indocyanine green loaded PLA nanofibers for in situ antimicrobial photodynamic therapy
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Lecture by Nobel Laureate Prof. Stefan Hell
iCANX on April 28th, 2022 at 14:00 (CEST)
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NEW abberior LIVE dyes
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Superresolution & Confocal Systems
Best-in-class imaging solutions for fluorescence superresolution and confocal microscopy will meet the most challenging requirements of your applications.
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