Abberior expression plasmids pQE31-rsEGFP2, 25 g

Item number: PR-3002-25UG

Abberior rsEGFP2 is the successor of rsEGFP and was generated for faster RESOLFT imaging. The switching speed was increased by a factor of 6 -1 0 compared to rsEGFP. This directly translates into faster RESOLFT superresolution imaging and brings down pixel dwell times to 75 - 400 s. Within this time span the typical 3-step RESOLFT process is applied ? activation, switching off and read-out. Reduced switching fatigue enables time lapse experiments with more image repetitions. The enhanced chromophore maturation (8 -10 times faster compared to rsEGFP) is advantageous for effective protein tagging in living mammalian cells.

rsEGFP2 is ideal for imaging with the commercial Abberior Instruments Expert Line RESOLFT Microscopes.

Selected references:

Grotjohann T et al. "rsEGFP2 enables fast RESOLFT nanoscopy of living cells" eLIFE 1:e00248, DOI: 10.7554/eLife.00248 (2012)

Schnorrenberg S et al. "In vivo super-resolution RESOLFT microscopy of Drosophila melanogaster" eLIFE 5. pii: e15567, DOI: 10.7554/eLife.15567 (2016)

Category: Photoswitchable

protein
quantity

$ 348.36

Excluding19% VAT. , plus shipping

Shipping time: 1 - 30 workdays



pQE31-rsEGFP2 (expression plasmid for E. coli)

Expression plasmid for the reversibly switchable fluorescent protein rsEGFP2; to be expressed in E. coli.

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