Abberior STAR 512

Item number: ST512

Abberior STAR 512 was developed for STED and confocal microscopy in the green spectral region. Abberior STAR 512 is highly photostable and bright green fluorescent dye. The dye works extremely well with Abberior Instruments Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with excitation lasers at 488 nm or 514 nm. Abberior STAR 512 can substitute dyes like Oregon Green® 514, ATTO® 514 or Alexa Fluor® 514. For STED microscopy, Abberior STAR 512 can be most efficiently used with STED Laser wavelengths between 590 - 610 nm. Best results are obtained with freshly prepared samples.

Abberior STAR 512 was successfully used in STED imaging and FCS experiments. Furthermore, Abberior STAR 512 serves well as a "donor" in a "donor - acceptor" dye pair used in FRET experiments.

Selected references:

Richardson DS et al. "SRpHi ratiometric pH biosensors for super-resolution microscopy" Nature Communications 8:577, DOI: 10.1038/s41467-017-00606-4 (2017)

Clausen MP et al. "A straightforward approach for gated STED-FCS to investigate lipid membrane dynamics" Methods 88:67-75, DOI: 10.1016/j.ymeth.2015.06.017 (2015)

Westin L et al. "Nanoscopic spine localization of Norbin, an mGluR5 accessory protein" BMC Neuroscience 15:45, DOI: 10.1186/1471-2202-15-45. (2014)

Category: Visible STED

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