Abberior STAR 470SXP

Item number: ST470SXP

Abberior STAR 470SXP is a long Stokes-shift dye optimized for STED and confocal microscopy in the orange spectral range. It shows a Stokes-shift of approx. 130 nm. As all long Stokes-shift dyes, it shows board excitation and emission band: It can be excited between 450- 500nm and emits between 600- 700nm. For STED microscopy, Abberior STAR 470SXP can be most efficiently used with STED Laser wavelengths between 750 - 800 nm. Abberior STAR 470SXP is designed and tested for multicolor STED microscopy in combination with Abberior STAR 580 and Abberior STAR 635P using a single STED wavelength (775 nm). Abberior STAR 470SXP can substitute dyes like Chromeo494 or DY-480XL. The hydrophilic properties of Abberior STAR 470SXP conjugates reduce unspecific binding of this dye and thus decrease undesired background fluorescence. Best results are obtained with freshly prepared samples.

Selected references:

Wilfling F et al."Arf1/COPI machinery acts directly on lipid droplets and enables their connection to the ER for protein targeting" eLife, DOI: 10.7554/eLife.01607 (2014)

Schill H et al. "4-Trifluoromethyl-Substituted Coumarins with Large Stokes Shifts: Synthesis, Bioconjugates, and Their Use in Super-Resolution Fluorescence Microscopy" Chemistry A European Journal 19(49):16556-65, DOI: 10.1002/chem.201302037 (2013)

Karlsson T et al."The Pseudomonas aeruginosa N-Acylhomoserine Lactone Quorum Sensing Molecules Target IQGAP1 and Modulate Epithelial Cell Migration" PLOS Pathogens 8(10):e1002953, DOI: 10.1371/journal.ppat.1002953 (2012)

Leica Microsystems recommendations for 2 Color applications

Category: Long Stokes-Shift

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