Abberior LIVE 590 is a dye designed for STED and confocal microscopy in living cells. Its molecular structure renders the dye strongly fluorogenic and facilitates its ability to penetrate cell membranes. It has an absorption maximum at 582 nm and can be effectively excited with a 561 nm laser. For STED, a depletion wavelength of 775 nm is recommended. Due to the enhanced biocompatibility Abberior LIVE 590 can be used in low concentration, reducing background and cytotoxicity effectively. Conjugates of Abberior LIVE 590 enable live cell imaging of DNA molecules or tubulin cytoskeleton.
Bucevicius J et al. "Enhancing the biocompatibility of rhodamine fluorescent probes by a neighbouring group effect" Chemical Science, DOI: 10.1039/D0SC02154G (2020)