Abberior LIVE 515

Item number: LV515

Abberior LIVE 515 is a dye designed for live-cell STED and confocal microscopy. Its molecular structure render the dye strongly fluorogenic and facilitates its ability to overcome cell membranes. It can be optimally excited between 500 and 530 nm. For STED, a depletion wavelength between 590 - 610 nm is recommended. Conjugates of Abberior LIVE 515 enable live-cell labeling of proteins and other biological structures e.g. lysosomes.

Selected references:

Butkevich AN et al. "Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells" Angewandte Chemie International Edition 55(10):3290-4, DOI: 10.1002/anie.201511018 (2016)

Butkevich AN et al. "Hydroxylated Fluorescent Dyes for Live-Cell Labeling: Synthesis, Spectra and Super-Resolution STED" Chemistry A European Journal 23(50): 12114-9, DOI: 10.1002/chem.201701216 (2017)

Butkevich AN et al. "Cell-Permeant Large Stokes Shift Dyes for Transfection-Free Multicolor Nanoscopy" Journal of the American Chemical Society 139(36):12378-81, DOI: 10.1021/jacs.7b06412 (2017)

Category: Live cell labeling

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Derivative: Azide Carboxylic acid Maleimide NHS ester
Quantity: 1 mg 50 g
Conjugate: pepstatin A
Excitation wavelength: 515 nm
STED wavelength: 595 nm

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