Abberior LIVE 510

Abberior LIVE 510 is a dye designed for live-cell STED and confocal microscopy. Its molecular structure renders the dye strongly fluorogenic and facilitates its ability to overcome cell membranes. It can be optimally excited between 485 and 520 nm. For STED, a depletion wavelength between 580 - 600 nm is recommended. Conjugates of Abberior LIVE 510 enable live-cell labeling of proteins and other biological structures e.g. mitochondria or tubulin cytoskeleton.

Selected references:

Butkevich AN et al. "Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells" Angewandte Chemie International Edition 55(10):3290-4, DOI: 10.1002/anie.201511018 (2016)

Butkevich AN et al. "Hydroxylated Fluorescent Dyes for Live-Cell Labeling: Synthesis, Spectra and Super-Resolution STED" Chemistry A European Journal 23(50): 12114-9, DOI: 10.1002/chem.201701216 (2017)

Lukinavicius G et al. "Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues" Chemical Science 9(13):3324-3334, DOI: 10.1039/c7sc05334g