Abberior CAGE 635

Item number: CA635

Abberior CAGE 635 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent red dye.

It can most effectively be excited from 620 to 645 nm. Abberior CAGE 635 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. It performs very well in the Leica GSD microscope and other superresolution microscopes as Nikon N-Storm.

Further, after photoactivation, Abberior CAGE markers can be tracked in confocal and epifluorescence microscopy to analyses molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.

Selected references:

Adelizzi B et al. "Painting Supramolecular Polymers in Organic Solvents by Super-resolution Microscopy" ACS NANO (5):4431-4439, DOI: 10.1021/acsnano.8b00396 (2018)

Johlin E et al. "Super-resolution imaging of light-matter interactions near single semiconductor nanowires" Nature Communications 7:13950, DOI: 10.1038/ncomms13950 (2016)

Aloi A et al. "Imaging Nanostructures by Single-Molecule Localization Microscopy in Organic Solvents" JACS 138(9):2953-6. doi: 10.1021/jacs.5b13585 (2016)

Category: Photoactivatable


starting from 200,00 €

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