Abberior CAGE 532

Item number: CA532

Abberior CAGE 532 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye. The dye is optimized for laser light at 532 nm.

Abberior CAGE 532 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. It performs very well in the Leica GSD microscope and other superresolution microscopes.

Further, after photoactivation, Abberior CAGE 532 can be tracked to analyze molecular dynamics such as diffusion, flow directions and velocities (e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore).

Selected References:

Belov VN et al. "Masked rhodamine dyes of five principal colors revealed by photolysis of a 2-diazo-1-indanone caging group: synthesis, photophysics, and light microscopy applications" Chemistry A European Journal 20(41):13162-73, DOI: 10.1002/chem.201403316 (2014)

Category: Photoactivatable

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Spectra

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