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Abberior CAGE 552 facilitates smFRET at physiological concentration levels.
Recently, Sijia Peng et al. (Angewandte Chemie Int. Ed. in English demonstrated) the advantages of single molecule photoactivation FRET (sm-PAFRET) using Abberior CAGE 552 as photo-switchable donor. Unlike conventional smFRET, this novel easy-to-implement technique allows to work close to physiological concentrations revealing new molecular mechanisms in the field of gene expression biology.
Abberior STAR dyes in Drosophila ovaries
Triple-stained Drosophila ovaries (Abberior STAR 580 phalloidin (green), Cut immunostain using Abberior STAR RED secondaries (red), DAPI (blue)). Imaged with an Abberior Instruments Expert Line microscope. Starting with a 10x dry objective, an overview of the organ is recorded. Switching to a 100x oil objective, we zoom into a confocal z-stack and further into a superresolved view detailing small clusters of Cut protein and actin strands. Sample prepared by J. Rehman, Abberior GmbH / Dr. H. R. Shcherbata, MPI for Biophysical Chemistry, Göttingen, Germany and imaged by J. G. Schloetel Abberior Instruments GmbH.
NEW: Abberior LIVE dye series
Combine the advantage of organic dyes - high photostability & brightness - with the ability of live-cell labeling:
- LIVE dyes are designed for cell permeability
- LIVE dyes are optimized for live-cell STED & confocal imaging
- Conjugates are tested for specific targets: tubulin, actin, lysosomes
- Also available as NHS ester, carboxylic acid, maleimide and azide
- Green & red fluorescent tags
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Confocal microscopy time lapse of actin filaments stained with LIVE 510 actin (jasplakinolide) in living human fibroblasts. Recorded were 480 frames at 60 sec intervals (total: 8 hours). The movie was recorded with an Abberior Instruments STED microscope.
Confocal microscopy time lapse of tubulin filaments stained with LIVE 510 tubulin (cabazitaxel) in living human fibroblasts. Recorded were 480 frames at 180 sec intervals (total: 24 hours). The movie was recorded with an Abberior Instruments STED microscope.
NEW: New conjugates & derivatives available for all Abberior superresolution dyes!
Abberior extends its product portfolio to allow a wider range of applications such as membrane labelling or biotin-streptavidin/Neutravidin binding assays.
Phospholipids: Try the new Abberior dye-labelled phospholipids DOPE, DPPE, C12 Sphingosyl PE (d17:1/12:0) for your membrane labelling experiments!
Biotin: All Abberior dyes are now available coupled to biotin to be used for fluorescent labelling of avidin/Streptavidin/Neutravidin-conjugated molecules.
Streptavidin/Neutravidin: Target your biotin-labelled molecule of interest with our new Abberior dye Streptavidin and Neutravidin conjugates.
Carboxylic acid: Make your own Abberior dye derivatives/conjugates with our carboxylic acids!
New secondary antibody specificities: We now offer goat anti-rat IgG, goat anti-guinea pig IgG and goat anti-chicken IgY conjugated to all Abberior dyes.
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Leading microscope vendors have succesfully tested Abberior dyes and provide references:
- Leica recommends Abberior
STAR dyes for STED. - STAR dyes for 2-color STED.
- CAGE dyes for Leica GSD.
- Zeiss have successfully tested Abberior dyes.
- Abberior CAGE dyes for Zeiss PALM.
- Nikon have successfully tested Abberior dyes.
- Abberior CAGE and FLIP dyes for 2-color Nikon N-STORM.
- Abberior Instruments recommends successfully tested STAR dyes for STED.
- STAR dyes for 2-color STED.