Abberior Instruments

Embedding media

  • Which embedding medium should i use for my application?

    Abberior offers different embedding media.

    Abberior Mount embedding media are aqueous embedding media with a refractive index in the range of 1.38. They are very convenient in terms of application. However, due to evaporation of contained water and polymerisation of the embedding medium (for Abberior Mount Solid), the refractive index will change over time.

    TDE Mounting media are 2,2'-thiodiethanol based embedding media with refractive indices in the range of 1.408 - 1.518. For many applications TDE Mounting media are superior to aqueous embedding media. Especially for demanding applications as 4Pi microscopy, 3D STED microscopy or isoSTED microscopy, because the refractive index will stay constant over time.

  • Does Abberior offer embedding media for STORM/ dSTORM/ GSDIM microscopy?

    In STORM, dSTORM and GSDIM microscopy frequently embedding media are used that have to be prepared directly before measurement.
    One reason for this is that compounds contained in these embedding media are degrading over time (i.e. enzymes, MEA and others). To this end we are not able to supply those embedding media. 

    For our CAGE and FLIP dyes conventional embedding media as PBS, MOWIOL, Abberior Mount and others may be used.

  • How should I seal mounted cover slips?

    Sealing of cover slips mounted with Abberior Mount embedding media can be either done with two-component silicone-glue Twinsil®. (Picodent, Wipperfürth, Germany) or clear nail polish (e.g.wet n wild Shine Nail Color Clear Nail Protector).

    Sealing of cover slips mounted with TDE Mounting media has to be done with clear nail polish (e.g.wet n wild Shine Nail Color Clear Nail Protector). Two-component silicone-glue Twinsil®. (Picodent, Wipperfürth, Germany) frequently does not polymerize completely.

    Please do not use colored nail polish or nail polish containing metallic particles.

  • CAGE dyes: Which embedding media are necessary for super resolution imaging?

    For our CAGE dyes conventional embedding media as PBS, MOWIOL, Abberior Mount and others may be used.

  • FLIP dyes: Which embedding media are necessary for super resolution imaging?

    For our FLIP dyes conventional embedding media as PVA, PBS, MOWIOL, Abberior Mount and others may be used.