back to previous Page

Accounting for the recent developments in the field of 2C-STED microscopy, we offer two different 2C packs using regular dyes.

The package contains Abberior STAR 580 and Abberior STAR 635P or Abberior STAR 600 and Abberior STAR 635P, which are testified to be used as 2-color STED dye pairs. The great advantage is to use two regular and very photostable dyes, no long-Stokes shift dyes are needed. Abberior STAR 635P is our favorite dye for STED microscopy in the infrared spectral region (Exc. 620 - 650 nm/ STED 750 - 780 nm). Abberior STAR 580 (Exc. 550 - 590 nm) and Abberior STAR 600 (Exc. 590 - 610 nm) enable 2-color STED with one laser wavelength at ~750nm - 775nm. The dye pairs are tested and verified for the Abberior Instruments 2C STED 775 QUAD Scan microscope.

Applications

As reference, please see a 2-color STED image with negligible crosstalk taken with our 2-color package Abberior STAR 580/STAR 600 and Abberior STAR 635P.

Comparison of a 2-color confocal (left) and the corresponding STED (right) microscopy image; overview (top panels), close-up (bottom panels). Labels are Abberior STAR 580 (TOM, green) and STAR 635P (giantin, red). Counterstaining: DAPI (blue).

 

Comparison of a 2-color confocal (left) and the corresponding STED (right) microscopy image; overview (top panels), close-up (bottom panels). Labels are Abberior STAR 600 (vimentin, purple) and STAR 635P (tubulin, grey). Counterstaining: DAPI (blue).

 

Comparison of a 2-color confocal (left) and the corresponding STED (right) microscopy image; overview (top panels), close-up (bottom panels). Labels are Abberior STAR 580 (Nup, green) and STAR 635P (lamin, purple).




Comparison of a 2-color confocal (left) and the corresponding STED (right) microscopy image. Labels are Abberior STAR 580 and STAR 635P.

  1. T. Müller et. al.  "STED Microscopy and its Applications: New Insights into Cellular Processes on the Nanoscale", ChemPhysChem, DOI: 10.1002/cphc.201100986  (2012)
  2. Kolmakov et al. "Red-Emitting Rhodamine Dyes for Fluorescence Microscopy and Nanoscopy", Chem. Eur. J., 16, 158 (2010)
  3. Bückers et al. "Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses", Opt. Expr., 19, 3130 (2011)
Comparison of a dual-color confocal (left) and the corresponding STED (right) microscopy image. Labels are Abberior STAR 580 (Nup, green) and STAR 635P (lamin, purple).

2C-Pack STED 775 IR -R-

520,00 EUR
19 % VAT plus. excl. Shipping costs
 Article and AmountOrder No.Net Price

* Other derivatives/conjugates on request.

This information is provided without liability and may be subject to change without prior notification.

Shipping time: Shipping time: on request

Newsletter subscription: