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Abberior STAR 635 is the latest dye development for STED imaging in the red spectral region. It features exceptional brightness and ultralow background, delivering stunning STED images. The dye is also recommended by Leica Microsystems for use in the Leica STED Ti:Sa microscope.
Abberior STAR 635 can substitute ATTO® 647N, AlexaFluor® 647, or Cy5®. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength around 750 nm is recommended.
Best results are obtained with freshly prepared samples.
Applications
Abberior STAR 635 particularly well suits the Leica TCS STED system and delivers high-resolution STED images with very low background signal due to its improved water solubility and exceptional brightness. The dye serves as an ideal partner for several 2-color STED packages.
Literature
- Leica Microsystems recommendations for 2-color applications
- A.P. Giese, J. Ezan, L. Wang, L. Lasvaux, F. Lembo, C. Mazzocco, E. Richard, J. Reboul, J. Borg, M.W. Kelley, N.Sans, J.Brigande and M. Montcouquiol, "Gipc1 has a dual role in Vangl2 trafficking and hair bundle
integrity in the inner ear", Development. 139(20):3775-85 (2012) - T. Müller, C.Schumann, A. Kraegeloh, "STED Microscopy and its Applications: New Insights into Cellular Processes on the Nanoscale", ChemPhysChem 13, 1986-2000 (2012).
- K. Kolmakov et al. "Red-Emitting Rhodamine Dyes for Fluorescence Microscopy and Nanoscopy", Chem. Eur. J. 16, 158 (2010)
- J. Bückers et al. "Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses", Opt. Expr. 19, 3130 (2011)
 |  | Data Sheet View |
Key Features
- Exceptionally bright and stable red fluorescent dye
- Ideal for Ti:Sa STED including the Leica TCS STED system
- High water solubility
| Article | Amount | Order No. | Net Price | ||
|---|---|---|---|---|---|
| Goat anti-mouse IgG |
|
2-0002-002-0 | € 295.00 | ||
| Goat anti-rabbit IgG |
|
2-0012-002-7 | € 295.00 | ||
| Maleimide |
|
1-0201-002-0 | € 295.00 | ||
| Maleimide |
|
1-0205-002-6 | € 1,230.00 | ||
| NHS ester |
|
1-0101-002-1 | € 295.00 | ||
| NHS ester |
|
1-0105-002-7 | € 1,150.00 | ||
| Azide |
|
1-0401-002-8 | € 295.00 | ||
| Phalloidin |
|
2-0205-002-5 | € 340.00 |
* Other derivatives/conjugates on request.
This information is provided without liability and may be subject to change without prior notification.