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Absorption & Fluorescence Spectrum

 

> Download spectral data (CSV format)

 

 Chemical Data

Structure:on request
Formula:on request
Molecular weight:795.6 g/mol (NHS ester)
Solubility:water, alcohols, acetonitrile, DMSO, DMF
Polarity:polar
Charge:-2 (when conjugated)
Purity:> 90 %


Photophysical Data

Absorption Maximum, λmax:501 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 86,000 M-1cm-1 (MeOH),
Correction Factor,
CF260 = ε260max
0.21
Correction Factor,
CF280 = ε280max:
0.11
Fluorescence Maximum, λfl:524 nm (PBS, pH 7.4)
Recommended STED
Wavelength, λSTED
585 − 605 nm
Fluorescence Quantum
Yield, η: 
0.96 (PBS, pH 7.4),
 
Fluorescence Lifetime, τ: 3.9  (EtOH)


Further Information

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Abberior STAR488 is the latest development for STED microscopy. It is a very bright green fluorescent dye that can be very effectively excited at the prominent 488 nm laser line. Abberior STAR 488 can substitute dyes like Oregon Green® 488, ATTO® 488 or Alexa Fluor® 488.

STED: The dye can most effectively be depleted in STED microscopy at 585-605 nm.
 

Applications

Below is animage taken with Abberior STAR488 in combination with Abberior STAR440SX in the Leica STED CW system as a reference.

 

The image below is taken with an Abberior STAR488 labeling and via using the Abberior EASYDOnut phaseplate.

Abberior STAR 488 is the dye of choice for excitation at 488 nm. Even more, the dye is particularly designed and tested for 2-color STED microscopy in combination with our STAR 440SX dye using a 590nm laser. The dye has been very successfully tested in the Leica 2-color TCS STED CW microscope.

Abberior STAR 488
Data Sheet View

Key Features

• STED dye of choice at 488 nm excitation
• Ideal depletion behaviour in STED microscopy ~590 nm
• 2-color labeling partner with STAR 440SX for 2-color STED microscopy

·   

Available Derivatives & Conjugates
Article Amount Order No. Net Price  
Goat anti-mouse IgG
500 µg
2-0002-006-8 € 295.00
Goat anti-rabbit IgG
500 µg
2-0012-006-5 € 295.00
NHS ester
1 mg
1-0101-006-9 € 295.00
NHS ester
5 mg
1-0105-006-5 € 1,150.00

* Other derivatives/conjugates on request.

This information is provided without liability and may be subject to change without prior notification.

Contact

Abberior GmbH / Hans-Adolf-Krebs-Weg 1

D-37077 Göttingen / Germany

Phone+49 551 30724 180
Fax:+49 551 30724 181
Email:info@remove-this.abberior.com
Web:www.abberior.com

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