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Absorption & Fluorescence Spectrum

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 Chemical Data

Structure:on request
Formula:on request
Molecular weight:595.57 g/mol (NHS ester)
Solubility:water, acetonitrile, MeOH, DMSO, DMF
Polarity:medium hydrophilic
Charge:0 (when conjugated)
Purity:> 90 %


Photophysical Data

Absorption Maximum, λmax:475 nm (MeOH),
477 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 30,400 M-1cm-1 (MeOH),
22,700 M-1cm-1 (PBS, pH 7.4)
Correction Factor,
CF260 = ε260max
0.69
Correction Factor,
CF280 = ε280max:
0.47
Fluorescence Maximum, λfl:609 nm (MeOH),
627 nm (PBS, pH 7.4)
Recommended STED
Wavelength, λSTED
740 − 770 nm
Fluorescence Quantum
Yield, η: 
0.80 (EtOH),
 
Fluorescence Lifetime, τ: 3.9  (EtOH)


Further Information

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Abberior STAR 470SX is the latest development of long-Stokes-Shift dyes for STED microscopy. The dye can be excited from 450 to 480nm. It can substitute dyes like Chromeo® 494. For STED, a depletion wavelength ~750 nm is recommended. It is therefore well suited for 2-color STED imaging as implemented in the Leica TCS STED Ti:Sa microscope. 

Applications


Abberior STAR 470SX is the dye of choice for long Stokes STED applications in the orange fluorescent regime. The dye is particularly designed and tested for 2-color STED microscopy in combination with our STAR 635 using a single STED wavelength. The dye is our recommendation for usage in the Leica TCS STED Ti:Sa 2-color system. For more information see our 2-color dye package section.

Literature

T. Karlsson et al., "The Pseudomonas aeruginosa N-Acylhomoserine Lactone Quorum Sensing Molecules Target IQGAP1 and Modulate Epithelial Cell Migration", PLoS Pathog. 8(10), e1002953, DOI:10.1371/journal.ppat.1002953 (2012).

Abberior STAR 470SX
Data Sheet View

Key Features

• Designed for STED microscopy@ ~750 nm
• Long Stokes’ shift (>130 nm) for 2-color applications
• Tested in the Leica TCS STED Ti:Sa 2-color system

·   

Available Derivatives & Conjugates
Article Amount Order No. Net Price  
Goat anti-mouse IgG
500 µg
2-0002-004-4 € 295.00
Goat anti-rabbit IgG
500 µg
2-0012-004-1 € 295.00
NHS ester
1 mg
1-0101-004-5 € 295.00
NHS ester
5 mg
1-0105-004-1 € 1,150.00

* Other derivatives/conjugates on request.

This information is provided without liability and may be subject to change without prior notification.

Contact

Abberior GmbH / Hans-Adolf-Krebs-Weg 1

D-37077 Göttingen / Germany

Phone+49 551 30724 180
Fax:+49 551 30724 181
Email:info@remove-this.abberior.com
Web:www.abberior.com

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