0
print
share
Dreiklang is a reversibly switchable fluorescent protein (RSFP) with a high fluorescence quantum yield and a large extinction coefficient. In contrast to all other known RSFPs today, Dreiklang has the unique property that besides two wavelengths used for switching between two different modifications – fluorescent and dark – a third wavelength can be used independently to read out the fluorescence. As a consequence, the fluorescence readout and photoswitching are completely decoupled processes.
A further improvement is the large number of switching cycles; Dreiklang allows >150 switching cycles before the fluorescence is reduced by 50%. Dreiklang proved a superior performance in superresolution imaging methods, such as RESOLFT, PALM or GSDIM.
The following scheme depicts the switching scheme of Dreiklang
| Excitation: | 515 nm |
| Photoactivation: | 405 nm |
| Photodeactivation: | 365 nm |
Applications
The superior features of Dreiklang make it a perfect marker for different approaches of superresolution microscopy such as RESOLFT microscopy as well as single-molecule switching (SMS) superresolution methods like PALM, STORM.
Literature
- J. Vaughan, X. Zhuang, "New fluorescent probes for super-resolution imaging", Nat. Biotechnol. 29, 880-881 (2011).
- T. Brakemann et al., "A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching", Nat. Biotechnol. 29, 942-947 (2011).
 |  | Data Sheet View |
Key features
- Unmatched switching contrast
- Superior performance in RESOLFT, PALM, GSDIM
- > 100 switching cycles
| Article | Amount | Order No. | Net Price | ||
|---|---|---|---|---|---|
| pQE31-Dreiklang expression vector |
|
3-0000-001-4 | € 290.00 |
This information is provided without liability and may be subject to change without prior notification.