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Abberior CAGE 500 is a masked dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye with spectral properties essentially identical to Abberior STAR 512. The dye performs e.g. very well in the Nikon N-Storm, particularly as a 2-color partner with CAGE552 or FLIP565.
Applications
Below images were acquired with a Nikon N-STORM microscope equipped with a quadband filter at the Nikon Imaging Application Center in Hamburg.
Abberior CAGE 500 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, Abberior CAGE markers can be tracked to analyse molecular dynamics such as diffusion, flow directions and velocities.
When released in close proximity to other (permanently fluorescent) dye molecules Abberior CAGE 500 can act as a FRET donor.
Literature
- V. N. Belov et.al. "Rhodamines NN: A Novel Class of Caged Fluorescent Dyes", Angew. Chem. Int. Ed. 49, 3520−3523 (2010)
- G. Y. Mitronova et.al. "New Fluorinated Rhodamines for Optical Microscopy and Nanoscopy", Chem. Eur. J. 16, 4477−4488 (2010)
 |  | Data Sheet View |
Key Features
- High brightness and photostability
- Ideal for PALM, STORM, GSDIM
- Well suited as 2nd PALM&STORM color
| Article | Amount | Order No. | Net Price | ||
|---|---|---|---|---|---|
| Goat anti-mouse IgG |
|
2-0002-101-0 | € 295.00 | ||
| Goat anti-rabbit IgG |
|
2-0012-101-7 | € 295.00 | ||
| NHS ester |
|
1-0111-101-8 | € 295.00 | ||
| NHS ester |
|
1-0115-101-4 | € 1,230.00 | ||
| Maleimide |
|
1-0211-101-7 | € 325.00 | ||
| Maleimide |
|
1-0215-101-3 | € 1,350.00 | ||
| Phalloidin |
|
2-0205-101-5 | € 340.00 |
* Other derivatives/conjugates on request.
This information is provided without liability and may be subject to change without prior notification.